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Wednesday, August 1, 2007

BT

Bt maize was developed in the United States primarily to control the European corn borer (ECB, Ostrinia nubilalis). This moth lays its eggs on maize leaves, damaging the plant. The larvae then tunnel into the leaves and the stalk. Into the autumn they migrate down the stalk and then spend the winter in the lowest part of the stalk or the top part of the roots. The stalks of the infected plants often break off.

The European corn borer was introduced to North America between 1910 and 1920 and then spread rapidly as a pest. In Europe it is found naturally on a number of different plants. Only one of the two corn borer strains in Europe actually attacks maize. This strain is native only to some parts of Europe; for example it is not found in northern Germany or Great Britain. However, the
ECB strain that attacks maize is slowly spreading northward and in Germany has been found as far as Brandenburg. In conventional agriculture ECB is usually controlled simply by ploughing the fields.

In autumn 2006, the approval to grow the GM maize MON810 in the EU will expire, which means the authorities will need to re-appraise this maize. Therefore Greenpeace has compiled the latest research from Germany and other countries, drawn up a list of open questions and clarified possible risks.

1 For Germany, the authors mostly used an analysis of research findings from a project of the German Ministry of Education and Research (BMBF 2006), "Safety research and monitoring for Bt maize cultivation 2001-2004", the results of which have only been published in part. The studies investigated MON810 and another type of GM maize (Bt 176) that is no longer being cultivated.

A report published in April 2006 by the European Commission shows that safety problems with GM crops have become more and more obvious over the last years (European Communities 2005).

The new data confirm this alarming finding. The wealth of indications that are available now show that the problems with GM maize are even more complex than originally assumed. The risks apply to the smallest soil organisms, to protected species such as butterflies and to beneficial insects such as bees and even extend to health risks for humans and animals.

The latest findings and the list of open questions clearly show that approval for commercial cultivation of the GM maize was granted prematurely and contradicts the precautionary principle that is part of EU legislation. The EU’s approval of the GM maize therefore must be withdrawn.

1. The cycle of the toxin in the environment
Normally the Bacillus thuringiensis toxin only exists in soil bacteria. This toxin has been used for many years to control agricultural pests. It is considered so harmless that it is even allowed in organic agriculture. But by genetically engineering the toxin into maize plants its characteristics have been changed fundamentally.

1. In nature, the toxin only exists in very low concentrations. If it is sprayed for pest
infestations, then it is used selectively and for a very short period of time.

2. The toxin in its natural form only kills certain insects. It comes in a non-active form
(protoxin) and it is not turned into the active form until it is in the insect’s gut.
Genetic engineering however changes the characteristics of the toxin:

1. It is produced in high concentration during the whole vegetation period of the plant and it is released through the roots, parts of the plant and pollen into the environment.

2. The toxin binds to soil particles and can survive in the soil for months. It can be passed on in the food chain and can even be passed through the gut of farm animals.

3. The toxin is not present in the inactive form, but in an active version. This changes the
range of possibly sensitive organisms.

4. Although the different toxic proteins are all called Cry1Ab, they are fundamentally
different from the natural protein, and they are different from each other.
Cultivating Bt maize creates a completely new cycle of distribution and concentration of Bt toxin in the environment and in the food chain. This has been confirmed by the latest research. Effects of Bt plants on the soil have only been investigated since the mid/late 1990s, i.e. only after Bt maize had already been cultivated in the USA, and only after Bt176 and MON810 had been approved for cultivation in the EU.
Many of the studies that have been published since the end of the 1990s on the topic of "Bt crops and soil" reveal unexpected effects, particularly negative environmental effects. These results also show that most areas have not been studied at all – and that nearly everywhere where research is done, indications of negative effects can be found.

How does the toxin get into the environment?
Bt toxins can get into the soil via different routes: as a living plant material (roots, Jehle 2005), as dead fine roots and root exudates during the growth period (Saxena et al. 1999, Saxena & Stotzky 2000), pollen (Losey 1999) that is washed into the soil, harvest residues (roots, stalks, leaves) after harvest (Tapp & Stotzky 1998, Stotzky 2000, Zwahlen et al. 2003b, Baumgarte & Tebbe 2005), and in animal excrement (Einspanier et al. 2004).

In recent years a series of studies with varying approaches was conducted to study the
persistence of Bt toxins in the soil, but there are only very few studies that investigate the amount and form of Bt toxin during and after the growth period. In 2005 it was still unknown how much toxin is actually exudated by the roots.
“To our knowledge, it is not known how much Cry1Ab protein is produced in the rhizosphere of Btmaize under agricultural practice and how much of that protein remains in the soil after harvesting” (Baumgarte & Tebbe 2005).
Apparently rather high toxin levels can be found in the soil close to the roots. Some of the toxin is found in the soil even months after the harvest, even though higher levels are found in the remaining plant residues:
“The amount of Cry1Ab protein in bulk soil of MON810 field plots was always lower than in the rhizosphere, the latter ranging from 0.1 to 10 ng/g soil. Immunoreactive Cry1Ab protein was also detected at 0.21 ng/g bulk soil 7 months after harvesting, i.e. in April of the following year. At this time, however, higher values were found in residues of leaves (21 ng/g) and of roots (183 ng/g), the latter corresponding to 12% of the Cry1Ab protein present in intact roots” (Baumgarte & Tebbe 2005).

Even though it is known that roots contain Bt toxin and can exudate it into the soil, this issue is not considered a factor at all in some risk assessments of Bt maize. For example, in the approval application for Bt maize 1507 that is currently pending at the EU, the Bt concentrations for different parts of the plant are given – but not that of the roots. Nevertheless, the EU authority EFSA gave a positive opinion for the commercial cultivation of this Bt maize.

The path of the toxin through roots, pollen and plant material is not the only path through which Bt toxin is released into the environment. Initial research into the degradation of Bt protein in the gut of cows shows that “remarkable” amounts of Bt toxin are found in the gastrointestinal tract, and that the animals’ faeces contains the toxin (Einspanier et al. 2004).

http://www.greenpeace.de/fileadmin/gpd/user_upload/themen/gentechnik/greenpeace_toxin_in_gm_maize.pdf




7:36 PM
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Fusarium

Currently, the available information on human health effects associated with fumonisins is not conclusive. However, based on the wealth of available information on the adverse animal health effects associated with fumonisins (discussed in this document and in the document entitled "Background Paper in Support of Fumonisin Levels in Animal Feed" prepared by FDA's CVM), FDA believes that human health risks associated with fumonisins are possible.

Based on the current available occurrence data, levels of fumonisins in human foods derived from corn are normally quite low. At the present time, FDA believes that these levels present a negligible public health risk. Nevertheless, FDA considers the fumonisin guidance levels to be a prudent public health measure during the development of a better understanding of the human health risk associated with fumonisins and the development of a long-term risk management policy and program by the agency for the control of fumonisins in human foods and animal feeds.

The recommended maximum levels for fumonisins in corn and corn products intended for human consumption (Table 1) are based on concerns associated with hazards shown primarily by animal studies. However, based on available information on the occurrence of fumonisins, FDA believes that typical fumonisin levels found in corn and corn products intended for human consumption are much lower than the recommended levels.


Total Fumonisins(FB1 + FB2 + FB3) parts per million (ppm)

Product:
1) Degermed dry milled corn products (e.g., flaking grits, corn grits, corn meal, corn flour with fat content of <>2.25 %, dry weight basis)
(4 ppm)

3) Dry milled corn bran
(4 ppm)

4) Cleaned corn intended for masa production (
4 ppm)

5) Cleaned corn intended for popcorn
(3 ppm)

http://www.cfsan.fda.gov/~dms/fumonbg3.html




6:58 PM
i love spongebob and patrick!

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